Analysis of the Media Essay Example | Topics and Well Written Essays - 2500 words

Analysis of the Media - Essay Example Occasionally, ridiculous plans will spring up to send them back to Britain – and these plans usually involve the radio transmitter (which is where the show gets its name) (BBC 2010). While all of this is going on, Rene is pretending to be his own identical twin (also named Rene), because he actually created a ruse about his own death to keep the SS away from him. This means that his wife is masquerading as his â€Å"widow,† who occasionally fields advances from potential suitors and who more often tries to entertain the patrons with her singing. Because her voice is so awful, though, customers either just leave or shove some cheese into their ears to avoid the maelstrom. In the meantime, Rene is carrying on with his waitresses; Edith generally finds out, but Rene can always talk his way out of the situation. This is helpful for him, because the gay German Lieutenant Gruber, as well as the attractive females in the communist resistance are always making advances toward h im. The episodes run consecutively, with the plot continuing from one episode to the next, but it is possible to watch each one and enjoy it independently. When each new episode begins, Rene shows up at the beginning, narrating the past events to the audience and bringing them up to date on his latest imbroglio (Brown 2008). When it comes to the structuring of the comedy, 'Allo 'Allo! emerges as part of a genre that appeared on both sides of the Atlantic Ocean in the decades after World War II. Hogan's Heroes, an American program, was one of the first television series that was able to use the Nazi machine as a source of comedy. The bumbling Colonel Klink, the commandant of Stalag 13, was constantly outwitted by his... The interplay between Basil and his wife, Sybil, serves up some of the most memorable comedic moments in the show. While she might agree, at least in principle, with his desire to make the hotel a more upscale establishments, she disagrees loudly (and sometimes violently) with his schemes. When Basil is trying a new idea, he usually gets Polly (the maid) or Manuel to help him – and to hide the truth from Sybil. Despite the anger that courses between them, though, it is clear that there is still some tenderness between them. â€Å"The Anniversary† is all about Basil's attempt to put together a surprise party for her (which goes awry when he pretends to forget the significance of the day, in order to make it even more of a surprise to her, and she storms out). However, Sybil is not a flat terror; instead, she often has the better hand when it comes to managing the hotel, particularly when there are difficult customers to be dealt with. There are other times, though, when she is talking loudly on the phone in the middle of a very busy scene at the hotel, and her braying laugh and loud voice make her a source o annoyance to others on the show.Manuel may be one of the most memorable characters from the show. From Barcelona, he still has only a limited grasp of British customs – and the English language.

Homogenized Mushroom Tissue Based Biosensors

Homogenized Mushroom Tissue Based Biosensors ABSTRACT Homogenized mushroom (Agaricus bisporus) tissue based biosensors by using plant tissue materials is a relatively new development in the biosensor technology. A simple in dip stick kind of visual ethanol biosensor that based on immobalised alcohol oxidase (AOX) homogenized from mushroom tissue onto polyaniline (PANI) film for ethanol (> 5% concentration ) detection in toiletries products. The colour going to change from green to blue due to response of biosensor to the ethanol and the change of colour can be seen by naked eyes. Regarding the enzymatic reaction of ethanol, acetaldehyde and hydrogen peroxide will produced, then PANI film is latter going to be oxidised. The method used to immobalise AOX onro the PANI film is by adsorption. Regarding immobilisation process, AOX solution need to deposit on the PANI film and then left it at room temperature within 30 minutes until it dry. Scan and analyse the changes film’s colour to obtain the biosensor’s response characteris tics toward the ethanol. The biosensor respond. Therefore, this simple visual biosensor is suitable for all-range-aged community to determine the safeness of certain toiletries products from the ethanol. Keywords : Biosensor; Alcohol Oxidase Ethanol; Mushroom Tissue; Polyaniline; Toiletries 6.0Â  EXPERIMENTAL 6.1Â  Chemicals 10-40 units/mg protein of Alcohol Oxidase or simply called (AOX) (A2404, EC1.1.3.13) which extract from mushroom (A. Bisporus). These mushroom can be bought at fresh market as culture vegetables. Before use make sure store it at 4Â °C. In order to immobalization use 225 bloom of gelatin from calf skin and 25.0% glutaraldehyde. Ascorbic acid, 2-propanol, D-glucose, n-butanol and many more chemicals needed can be purchased from Sigma, St. Louis, USA. Aniline with AR-grade, gallic acid (G7384) and l-cysteine (W326305) can be purchased from Sigma Aldrich (Saint Louis, MO, USA). Ethanol which contain >99.5%, methanol, orthophosphoric acid (85%) and sodium hydroxide (pellets) can be delivered by Merck (Nottingham, UK). All needed chemicals are from commercial source which in analytical grade. Millipore Direct-QTM 5 purification system provide the Milli-Q water. Prepare ethanol’s stock solutions in 0.1 M phosphate buffer at suitable pH daily and store it in refrigerator at 4 Â °C. For pH studies, 0.1 M of the phosphate buffer solutions with pH values between 4 and 8 were can be used and to measure the pH value, use commercial glass electrode and pH-meter (model 9318, Hanna Instruments, Woonsocket, RL, USA) and calibrate it at the pH values of 4.00, 7.00 and 9.00. 6.2Â  Homogenization of Mushroom Tissue Homogenize 200 mg mushroom tissue in the 400ml phosphate buffer. Mix this 300 ml homogenate and 10 mg gelatin. Incubate it for 5 minutes at 38Â °C to make sure the gelatin is dissolved. 6.3Â  Preparation of Polyaniline (PANI) Film In order to prevent bumping in the aniline, purify the aniline with vigorous and rapid stirring by distilled under vacuum. Prepare PANI dispersion as nanofibre and Huang and Karner (2006) already mention the method to should to be used to build it. Mix 3.2 mmol or 0.3 g of purified aniline with 10 mL of 1.0M Hydrochloric Acid (HCL). Mix 0.8 mmol or 0.18 g of Ammonium peroxydisulfate into another 10 mL aliquot acid solutions. Add aniline-acid to oxidant and these two solution will mix rapidly within 30 seconds and then allow it to react in undisturbed overnight condition. On the next day, wash the polyaniline by using water and centrifuged. Supernatant liquor with pH 3.3 and strong green colour will form and it is indicate as the PANI particles which can be observed after several times of washing. Any remaining particles with size larger than 1ÃŽ ¼m must be removed before casting by passing the dispersion through a 55-mm glass fiber filter (Whatman GFA, Kent, UK) which is attach to va cuum source. Cast directly PANI dispersion on a substrate of polystyrene and then, left the thin film of PANI that on the sheet of polystyrene in the dark to dry after cut it into individual in 10mm2 size. Next, store that ready film at 4Â °C. The thickness must be 0.7ÃŽ ¼m and use SEM images to determine it. To make sure the thickness of the film is always in the same magnitude order, it must be determined routinely. Then, choose 0.7 ÃŽ ¼m thickness of PANI film to use for the further experiment result in good of PANI film fabrication reproducibility. 6.4Â  Enzyme Immobilization Immerse the PANI film in 0.1 M phosphate buffer which have pH 7.0 to make sure the condition of PANI film is at natural condition which means at pH 7.0. After that, deposit appropriate concentration about 10 ÃŽ ¼L of AOX solution which is from homogenate of mushroom tissues and phosphate buffer on the PANI film and left it to dry within 30 minutes. For the further use, store this PANI film with immobilised AOX at 4 Â °C. 6.5Â  Biosensor Construction Construct a dip stick test visual biosensor of PANI film with immobilized AOX as Figure 1, connect AOX/PANI film with a handle which made by cellulose paper or can use transparent plastic tape. To way to use this this dip-stick format visual biosensor by just dipping this kind of biosensor into the toiletries sample solution for a several seconds (Â ±5 s), then the change of colour can be seen by baked eye if the concentration of ethanol is >5% since that only amount allowed to be in toiletries products and use image analysis for the quantitative measurement change of colour. (a)(b) Figure 1: (a) dip stick format of biosensor (b) dip biosensor into toiletries sample solution (Kuswandi et.al,2014) 6.6 Colour Change Recording See the change of colour by naked eye during alcohol detection since this biosensor is kind of visual mode. Use scanner for example Canon, Cano Scan, Japan and Tokyo for quantification of colour measurements. The presence of ethanol exceed 5% of concentration in toiletries sample solutions will change the colour of the biosensor from green to blue. The detection can be done by dipped the biosensor which in the form of dip stick test in the sample solutions in 5 seconds. Use ImageJ program which can be used as online applet, free download application or can be used in any computer together with Java 5 (Dougherty, 2009 Rueden et.al, 2007) in order to assess the colour after it has been scanned. The purpose s to determine the mean RGB colour value. 7.0Â  EXPECTED RESULT The expected result from this research is the colour of dip stick AOX/PANI film biosensor will change from green to blue if there presence of >5% of ethanol in toiletries products after the biosensor is dipped into the toiletries sample solutions for 5 seconds. Since this is the visual mode biosensor, the change of colour can easily seen by naked eyes. On the hand, use scanner (Canon, Cano Scan, Japan, Tokyo) for quantitative colour measurement and then use ImageJ program to assess the colour change of biosensor and to determine the mean RGB colour value. (Collins, 2007)

A Dream with Deceit Essay -- American Argumentative Persuasive Essays

A Dream with Deceit In a world of uncertainty and disappointment, many people welcome the promise of happiness by any definition. In response to this, society offers the "American Dream," a guarantee of success through hard work and perseverance, as a path to contentment. Corporate America cunningly markets the "American Dream" to the public, and as a result the allure of wealth and status dictates the lives of many Americans. The elite and large corporations intentionally feed the idea of an "American Dream" to the general public because they depend on its widespread acceptance to maintain their undisputed power. The media has become a powerful tool for corporations to spread the ideals of the "American Dream" throughout the population. Television and newspapers, two specific channels of the media, have a vast influence over the people that the "American Dream" targets. The power that the media has over the general population is strong, influencing society's ideals and view of the "American Dream" through methods equivalent to brainwashing. Society continues to uphold a destructive "American Dream," giving people a false perception of success and ignoring the reality of limited opportunity that exists today. Society has imposed the "American Dream" on individuals for as long as the United States has been in existence. People are manipulated into believing that hard work and perseverance will lead to their ultimate success in life. The government, the media, and corporations alike create and exploit the "American Dream" as a system of mass control. Greed propels the dream by adding to the power it hold over individuals. Society is told that everyone should be striving for success, which is defined a... ...bert Cullen, and Bonnie Lisle, Boston: Bedford / St. Martins, 2001. 305-310. Dalton, Harlon L. "Horatio Alger." Rereading America. Eds. Gary Colombo, Robert Cullen, and Bonnie Lisle, Boston: Bedford / St. Martins, 2001. 311-317. Fallows, James. "The Invisible Poor." Rereading America. Eds. Gary Colombo, Robert Cullen, and Bonnie Lisle, Boston: Bedford / St. Martins, 2001. 356-369. "Money and Success: The Myth of Individual Opportunity." Rereading America. Eds. Gary Colombo, Robert Cullen, and Bonnie Lisle, Boston: Bedford / St. Martins, 2001.295-297. Mantsios, Gregory. "Myths and Realities." Rereading America. Eds. Gary Colombo, Robert Cullen, and Bonnie Lisle, Boston: Bedford / St. Martins, 2001. 318-333. Terkel, Studs. "Stephen Cruz." Rereading America. Eds. Gary Colombo, Robert Cullen, and Bonnie Lisle, Boston: Bedford / St. Martins, 2001. 335-339.

Comparing How Two Short Fiction Writers Effectively Convey a Common Theme Essay

â€Å"A Visit of Charity† by Eudora Welty and â€Å"The Use of Force† by William Carlos Williams are both engrossing short stories featuring two young female characters who must face an ailment. Eudora Welty touches on a social ailment, using an indifferent teenage girl as takeoff point, and portrays the abandonment of the elderly in a home that is as cold as the treatment they are subjected to. On the other hand, Williams tackles a physical ailment, and he depicts how a medical practitioner will go to great lengths not to neglect his duty, even if he encounters resistance. Welty takes on a serious, somewhat mocking tone, while Williams is thoroughly amusing and almost comical, yet very realistic. In Welty’s short story, a teenage named girl Marian is the protagonist who temporarily leaves the confines of her cloistered existence to visit a home for the aged to fulfill a requirement for Campfire Girls, a group that seeks to develop character in the young.   Instead of gaining the expected social benefits from the visit, Marian ironically develops great uneasiness and retreats. Both short story titles reflect their themes and plot, although the titles may have other implications. â€Å"Visit† in Welty’s short story implies Marian’s fleeting and superficial encounter   with elderly women in the institution. Another possible meaning of the title, â€Å"A Visit of Charity† is that in the world of   apathetic young people, there may be an instance when life offers an opportunity to look beyond themselves and show helpfulness or compassion for fellow human   beings. The title may be seen then as a case of Charity paying Marian a visit by offering her the opportunity to help or give solace or relief to the needy.    Being young and preoccupied with her personal agenda, though, she fails, as most young people do, to enrich herself from a special opportunity to reach out and be of service to others. As for â€Å"The Use of Force† by William Carlos Williams, the title at first glimpse conjures a serious or frightening situation like the application of brute force, a common subject in many literary works. In â€Å"The Use of Force† by Williams, we see instead the firm hand   of a physician who responds to the call of duty as he overcomes the temporary setback of an unwilling and naughty, young patient who resists him. The setting, characters, events and situations in both short stories come alive through the use of vivid language. When Welty describes the home for the aged as   having walls that bounce off † the winter sunlight like a block of ice† (Welty 245) and corridors that â€Å"smell like the interior of a clock† (Welty 246), she lets readers in on the pitiable plight of the elderly women who endure not just the poorly illuminated and unkempt dwelling place but also the neglect and contempt of people entrusted to care for them, or society as a whole. Welty uses a lot of similes like these as the story progresses. William likewise uses figures of speech like personification as he amusingly narrates his experience with the impish Mathilda, the sick child with overprotective parents who are easily swayed by her whims but who care for her well-being. Williams, for instance describes the antagonist as follows: â€Å"The child was fairly eating me up with her cold, steady eyes† (Stuber). There are also symbolisms in each short story.   In â€Å"A Visit of Charity,† the potted plant that Marian brings to the home for the aged is just a thing or gift that is expected of a visitor.   In the same way, the elderly ladies are placed in the home because it is the right thing to do, even if they are not given the genuine warmth and attention they deserve as human beings. In â€Å"The Use of Force,† the parents of the sick child represent a bygone era, when medicine was not yet fully developed as a science and old-fashioned remedies were resorted to.   The doctor may therefore represent the western world’s modern man or â€Å"savior† of those afflicted with illness. In â€Å"A Visit of Charity† by Eudora Welty, the main character desires or concentrates on her own personal advantage.   In contrast, â€Å"The Use of Force† by William Carlos Williams depicts a doctor’s selfless concern to be of help to his patient. In both short fiction stories, the characters interact and engage in dialogue as part of a first-time encounter. The dialogue breathes life to the personalities of the characters, allowing readers to visualize and relate to them better, while also advancing the plot and making the story even more interesting. Both authors lend credibility to the main characters’ views by first establishing their age, gender. task or occupation, and the social class they represent as seen from their mode of dressing and speech. Overall, both William Carlos Williams and Eudora Welty are masters of the short story form who are effectively able to convey important social themes through imagery and characterization, among other elements of short fiction. Works Cited Stuber, Irene. â€Å"The Use of Force by William Carlos Williams (1883-1963).† Classic Short Stories. 1995. 17 July 2008 . Welty, Eudora. â€Å"A Visit of Charity.† Modern Satiric Stories: The Impropriety Principle. Ed. Gregory Fitzgerald. Illinois: Scott, Foresman and Company, 1971. 245-246.

Film Opinion Essay

When I decide to drive to the movie theater or to sit down and enjoy a film, several factors play in my choice of movie. Most of the time, it depends on the mood that I am in. Am I in the mood for something light and funny? Or do I want to submerge myself into a detail-oriented film such as Inception? I take into consideration whether I have enjoyed the actors in previous films, and if I think they will provide the entertainment I am looking for. Has the trailer engaged me long enough to make me interested in the film? Or has the advertising flooded me with information that makes me feel as though the film will be another flop and not worth my time? These are all questions I ask myself when I start to think about watching a movie. Generally, I don’t take movie critic reviews into consideration because I have been disappointed several times in the past, but I will listen to what friends have to say about their experience with the movie. I enjoy most films across all genres. I enjoy when the stories are detailed, and provide a history of the characters, whether through flashbacks or dialogue. I prefer comedies to be humorously dark, sarcastic, or dry. With dramas or action flicks, I prefer for the protagonist to have some tragic faults that lead to their outcome or victory to be sweeter or to justify a better cause than them. I enjoy dialogue-heavy films with actors who can portray the character in a way that makes me believe in them. The recent run of comic-based movies is thoroughly enjoyable to me, due to the rich storytelling and deep backstories from the comic books that the movies accompany. Seeing those characters given life by actors that pull off the mannerisms and attitudes helps ensnare new fans into the world of comics. Science fiction films draw me in because I have always been intrigued by the idea that our technological advancement will eventually cause issues that could lead to humanity’s downfall. When I can watch movies over and over again and find new, interesting subplots or Easter eggs, it makes the experience more rewarding. I do not enjoy films with plotlines that do not make sense, create a story too difficult for the medium to translate properly for the audience, or when movies start plotlines but do not see them through. When watching horror films, slasher films do not pique my interest as much as psychological or religious horror films do. I try to stay away from the general run of the mill action films that come out every summer, that tend to have the same formula as a lot of other action movies. In rare instances, the soundtrack of a film will turn me away if it does not fit the mood and movement of the film. This makes them very predictable and unenjoyable to me. I also find it difficult to watch movies that are based on books but take too many liberties and do not follow the book’s storyline. All in all, I enjoy watching movies and do so several times a week if I have the time. Whether it be new films or favorites that I have watched many times, it is always good to escape from reality for a short time and to relax. Movies have always been a good conversation piece for my circle of friends, and are usually what we choose to do when we get together. I find that I have no problem sitting down and starting any movie at home to see if it will capture my interest, but when it comes to paying for a ticket, I am more picky because I do not want to feel as though I wasted my money.